Coding

Part:BBa_K3807012:Design

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


gRNA molecular ruler 7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

enCas9 protospacer designed to bind at position 648 merged to enCas9 gRNA body that we took from the original pEvolvR plasmid

Source

Amplified from Addgene plasmid #113077 and modified with 20nt spacer sequence

References

Halperin et al, 2018